Degradation of ancient DNA

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Degradation of ancient DNA

Since the appearance of the first reports describing the isolation and cloning of DNA from a 140-year-old preserved quagga [1] and from a 2 400 year-old Egyptian mummy [2], ancient DNA has been extracted from a number of organisms preserved in a variety of ways. Sources include preserved museum specimens, frozen mammoths, a 17 million year-old Magnolia leaf, and a 130 million yearold weevil ent...

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Ancient DNA from Human and Animal

Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...

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Ancient DNA

Ancient DNA is a field of molecular evolutionary biology that usesDNA sequence data recovered frompoorly preserved organisms, usually deceased for hundreds to hundreds of thousands of years. Ancient DNA data can provide unique snapshots in time to better understand how populations and species evolve. The field was born in the early 1980s,when thefirst ancientDNAsequences were recovered from pre...

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Epigenetics of Ancient DNA

Initially, the study of DNA isolated from ancient specimens had been based on the analysis of the primary nucleotide sequence. This approach has allowed researchers to study the evolutionary changes that occur in different populations and determine the influence of the environment on genetic selection. However, the improvement of methodological approaches to genome-wide analysis has opened up n...

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Bayesian inference of errors in ancient DNA caused by postmortem degradation.

Methods for extracting and amplifying sequences using ancient DNA (aDNA) can be prone to errors caused by postmortem modifications of the DNA strand. A new statistical method is developed for predicting errors in aDNA sequences caused by such processes. In addition to the canonical DNA substitution model parameters, a discrete Markov chain is used to describe nucleotide substitutions occurring ...

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ژورنال

عنوان ژورنال: Current Biology

سال: 1996

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(02)00460-8